Coding
Part:BBa_K3799026:Design
Designed by: Shubhamay Das, Tanya Ivanov, Franklin Zheng Group: iGEM21_IISER_Kolkata (2021-10-19)
Type III-A CRISPR-Cas effector ribonuclease---Csm6
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For easy protein purification, his tag with a TEV cut site is added in the 5' end of the coding sequence.
Source
Csm6 is encoded by several bacteria that use Type III CRISPR-Cas systems. The sequences we use in our characterization are derived from Enterococcus italicus.
https://www.uniprot.org/uniprot/E6LHV2